Fig. 5
ExosomeMIF inhibited cardiomyocyte senescence by delivering LncRNA–NEAT1. a DiI-labeled exosome transfer is shown. b LncRNA–NEAT1 mRNA in cardiomyocytes was detected using qRT-PCR. *P < 0.05 vs control; ▲P < 0.05 vs exosomeMIF in one-way analysis of variance, n = 3. SiRNA was used to silence LncRNA–NEAT1 in MSCs. c QRT-PCR was applied to test LncRNA–NEAT1 mRNA in cardiomyocytes after LncRNA–NEAT1 silencing in MSCs. *P < 0.05 versus Control; ▲P < 0.05 versus exosome;○P < 0.05 versus exosomeMIF+siRNA−LncRNA−NEAT1 in repeated measures ANOVA, n = 3. d Cell cycle distribution was analyzed. e, f p27 and p16 mRNA levels were analyzed using qRT-PCR. g Representative images of SA-β-gal staining. h Percentage of β-gal-positive cells. i Telomere length was analyzed by qRT-PCR. j Relative telomerase activity was measured. *P < 0.05 versus Control; ▲P < 0.05 versus Dox; ○P < 0.05 versus Dox + exosomeMIF+siRNA−LncRNA−NEAT1 in repeated measures ANOVA, n = 3