Fig. 6
LncRNA–NEAT1 transferred by exosomeMIF sponged miR-221-3p against cardiomyocyte senescence. a Binding sites of LncRNA–NEAT1 and miR-221-3p. b Dual-luciferase reporter. *P < 0.05 versus miR-221-3p mimic in the WT group in repeated measures ANOVA, n = 3. c NEAT1 is associated with miR-221-3p, confirmed by biotin-based pulldown assay; *P < 0.05 versus Bio-NC in repeated measures ANOVA, n = 3. d miR-221-3p mRNA levels were analyzed by qRT-PCR. *P < 0.05 versus Control; ▲P < 0.05 versus Dox; ○P < 0.05 versus Dox+ exosomeMIF+siRNA−LncRNA−NEAT1 in repeated measures ANOVA, n = 3. e Overexpression effect was validated by qRT-PCR. *P < 0.05 versus miR-221-3p mimic in repeated measures ANOVA, n = 3. f Cell cycle distribution was analyzed. g, h p27 and p16 mRNA levels were analyzed using qRT-PCR. i Representative images of SA-β-gal staining. j Percentage of β-gal-positive cells. k Telomere length was analyzed by qRT-PCR. l Relative telomerase activity was measured. *P < 0.05 vs Control; ▲P < 0.05 vs Dox; ○P < 0.05 vs Dox+ exosomeMIF + miR-221-3p mimic in repeated measures ANOVA, n = 3