Fig. 7
MiR-221-3p shuttling by exosomeMIF reversed senescence through targeting Sirt2. a–c QRT-PCR (a) and western blot analysis (b, c) tested the siRNA-mediated transfection efficiency. Each column represents the mean ± SD from three independent experiments. *P < 0.05 versus siRNA-Sirt2 in repeated measures ANOVA, n = 3. d Cell cycle distribution was analyzed. e, f p27 and p16 mRNA levels were analyzed using qRT-PCR. g Representative images of SA-β-gal staining. h Percentage of β-gal-positive cells. i Telomere length was analyzed by qRT-PCR. j Relative telomerase activity was measured. *P < 0.05 versus Control; ▲P < 0.05 versus Dox; ○P < 0.05 versus Dox + exosomeMIF + siRNA-Sirt2 in repeated measures ANOVA, n = 3