Skip to main content

Table. 1 Primer sequences

From: Exosomal LncRNA–NEAT1 derived from MIF-treated mesenchymal stem cells protected against doxorubicin-induced cardiac senescence through sponging miR-221-3p

Genes Sequences
p27 F: 5′-CCTGGAGCGGATGGACGCCAGACA-3′
R: 5′-CACCAAATGCCGGTCCTCAGAGTT-3′
p16 F: 5′-TTG GCC CAA GAG CGG GGA CA-3′
R: 5′-GCG GGC TGA GGC CGG ATT TA-3′
p21 F: 5′-TCCACAGCGATATCCAGACA-3′
R: 5′-GGACATCACAGGATTGGAC-3′
Sirt1 F: 5′-TTGGCACC GATCCTCGAAC-3′
R: 5′-CCCAGCTCCAGTCAGAACTAT-3′
Sirt2 F: 5′-TGAATGGCACCTACAGAGAC-3′
R: 5′-CAAAGGCATTATGGTAGGGC-3′
Sirt6 F: 5′-AGTTCGACACCACCTTTGAG-3′
R: 5′-CGTACTGCGTCTTACACTTG-3′
LncRNA–NEAT1 F: 5′-TTGGGACAGTGGACGTGTG-3′
R: 5′-TCAAGTGCCAGCAGACAGCA-3′
miR-221-3p F: 5′-GGGAAGCTACATTGTCTGC-3′
R: 5′-CAGTGCGTGTCGTGGAGT-3′
Telomere length F: 5′-TGAAAGTAGAGGATTGCCACTG-3′
R: 5′-AGCCAGAACAGGAACGTAGC-3′
GAPDH F: 5′-GGA GCC AAA AGG GTC ATC AT-3′
R: 5′-GTG ATG GCA TGG ACT GTG GT-3′
U6 F: 5′-CTCGCTTCGGCAGCACA-3′
R: 5′-AACGCTTCACGAATTTGCGT-3′
siRNA–LncRNA–NEAT1 5′-TACCATCAGCCTTTAG-3′
siRNA–LncRNA-NT 5′-AACACGTCTATACGC-3′
siRNA–Sirt2 5′-GCAGCUUGUGUGAGCUCAATT-3′
siRNA-NT 5′-UUGAGCUCACACAAGCUGCTT-3′