|
p27
|
F: 5′-CCTGGAGCGGATGGACGCCAGACA-3′
|
|
R: 5′-CACCAAATGCCGGTCCTCAGAGTT-3′
|
|
p16
|
F: 5′-TTG GCC CAA GAG CGG GGA CA-3′
|
|
R: 5′-GCG GGC TGA GGC CGG ATT TA-3′
|
|
p21
|
F: 5′-TCCACAGCGATATCCAGACA-3′
|
|
R: 5′-GGACATCACAGGATTGGAC-3′
|
|
Sirt1
|
F: 5′-TTGGCACC GATCCTCGAAC-3′
|
|
R: 5′-CCCAGCTCCAGTCAGAACTAT-3′
|
|
Sirt2
|
F: 5′-TGAATGGCACCTACAGAGAC-3′
|
|
R: 5′-CAAAGGCATTATGGTAGGGC-3′
|
|
Sirt6
|
F: 5′-AGTTCGACACCACCTTTGAG-3′
|
|
R: 5′-CGTACTGCGTCTTACACTTG-3′
|
|
LncRNA–NEAT1
|
F: 5′-TTGGGACAGTGGACGTGTG-3′
|
|
R: 5′-TCAAGTGCCAGCAGACAGCA-3′
|
|
miR-221-3p
|
F: 5′-GGGAAGCTACATTGTCTGC-3′
|
|
R: 5′-CAGTGCGTGTCGTGGAGT-3′
|
|
Telomere length
|
F: 5′-TGAAAGTAGAGGATTGCCACTG-3′
|
|
R: 5′-AGCCAGAACAGGAACGTAGC-3′
|
|
GAPDH
|
F: 5′-GGA GCC AAA AGG GTC ATC AT-3′
|
|
R: 5′-GTG ATG GCA TGG ACT GTG GT-3′
|
|
U6
|
F: 5′-CTCGCTTCGGCAGCACA-3′
|
|
R: 5′-AACGCTTCACGAATTTGCGT-3′
|
|
siRNA–LncRNA–NEAT1
|
5′-TACCATCAGCCTTTAG-3′
|
|
siRNA–LncRNA-NT
|
5′-AACACGTCTATACGC-3′
|
|
siRNA–Sirt2
|
5′-GCAGCUUGUGUGAGCUCAATT-3′
|
|
siRNA-NT
|
5′-UUGAGCUCACACAAGCUGCTT-3′
|
