Fig. 3

HK2 knockdown induced by HK2 shRNA-loaded nanoparticles in ovarian cancer cells. a Relative HK2 mRNA levels according to qRT-PCR and b HK2 protein levels according to western blotting in A2780 and A2780CP cells treated with different HK2 shRNAs for 24 h and 48 h, respectively. The concentration of the HK2 shRNA plasmid was 1.0 μg/ml. The transfection reagent was added to the culture medium. c Relative HK2 mRNA levels and d HK2 protein levels in A2780 and A2780CP cells treated with HK2 shRNA-loaded nanoparticles for 24 h and 48 h, respectively. The nanoparticles were diluted to a plasmid-equivalent concentration of 1.0 μg/ml. The transfection reagent was added in the lipo-shHK2 group but not added in other groups. e Cellular HK2 enzyme activity changes in A2780 and A2780CP cells treated with HK2 shRNA-loaded nanoparticles. *P < 0.05, **P < 0.01 vs. control