Fig. 4

HK2 knockdown induced by DFPP-shHK2 facilitated a shift in the glucose metabolism pattern of ovarian cancer cells. A2780, A2780CP and H1299 cells were treated with DFPP-shHK2 and cisplatin alone or in combination for 24 h. The concentration of cisplatin was 2.5 μg/ml. DFPP-shHK2 was used at a plasmid-equivalent concentration of 1.0 μg/ml. The OCR was measured using the Seahorse XF96 analyzer in A2780 (a), A2780CP (b) and H1299 (c) cells. Oligomycin, FCCP, rotenone and antimycin A were added to challenge the cells. d The OCR changes showing FCCP-stimulated oxygen consumption compared to the baseline. The ECAR was measured in A2780 (e), A2780CP (f) and H1299 (g) cells challenged by glucose, oligomycin and 2-DG. h The ECAR changes showing oligomycin-stimulated glycolysis compared to the baseline. i Glucose level in medium. j Lactate level in medium. k Cellular ATP levels. *P < 0.05, **P < 0.01 vs. control