Fig. 4
Uptake of cancer cell-derived Reg-EVs and Br-EVs in human THP-1 monocytes. Breast cancer Reg EVs were derived from MDA-MB-231 cells and breast cancer Br-EVs from MDA-MB-231-BrM2-831 cells. Melanoma Reg-EVs were derived from A375 cells and melanoma Br-EVs from M12 melanoma brain metastases cells. Cell culture media were depleted of exogenous EVs and lipoproteins. a Flow cytometry and corresponding schematics of THP-1 cells incubated with fluorescently labeled breast cancer EVs. b Flow cytometry and corresponding schematics of THP-1 cells incubated with fluorescently labeled melanoma EVs for four hours. c Flow cytometry and corresponding schematic of HBMECs incubated with breast cancer EVs for four hours. LDL dose, 500 μg/1010 EVs. Data are presented as mean ± SD of three replicates. Statistical analysis was done using two-way ANOVA with post-hoc pairwise comparisons calculated with a Tukey’s test. ***p < 0.001; ****p < 0.0001