Fig. 4

Single-cell tracking analysis of early biofilm formation. a Image sample of the adherent bacteria during the early stage of biofilm formation in M9 and TSB medium. b Single-cell tracking was performed to quantify the amount of adherent bacteria, the generation of new ones, and their release from the surface. The data is plotted as the mean count of bacteria and events per field of view (FOV) (lines) with standard deviation (shaded area) based on three locations with a sampling rate of 10 min