Fig. 1

AntimiR-375 and antimiR-141 reduce tumor growth of prostate cancer in vitro and in vivo. a LNCaP prostate cancer cells were cultured under standard culture conditions and exposed to antimiR-375 or antimiR-141. Cell proliferation was measured using the colorimetric WST-1 assay. b PC3 and DU145 prostate cancer cells were transfected with antimiR-375 or antimiR-141 and grown at low density and stained with Hematoxylin. c, d DU145 prostate cancer cells were transfected with antimiRs and analyzed for anchorage-dependent (c, on plastic) and anchorage-independent (d, in soft agar) colony formation capacity. e LNCaP, PC3 and DU145 prostate cancer cells were transfected with antimiRs as above and caspase activity was measured as a marker for apoptosis induction