Fig. 2

Nanoparticle complex stability and transfection rates. a For the determination of complexation efficacy by agarose gel electrophoresis, 0.5 µg antimiR were complexed with PEI at the different weight ratios. The complexes were mixed and separated on a 2% agarose gel pre-stained with Sybr™ Gold. b Complex stabilities were measured by heparin displacement assay. c PEI/antimiR complex sizes were determined by Dynamic Light Scattering (DLS). d Transmission electron microscopy (TEM) of PEI/antimiR complexes. e,f Uptake of PEI complexes containing Cy3-labeled antimiR was analyzed by flow cytometry and confocal microscopy