Fig. 4

miR-150 drives melanoma growth in vivo. a TaqMan^® low-density arrays were used to measure the expression of 667 human miRNAs in a set of melanocytes, primary melanomas and cutaneous melanoma metastases. Relative expression of indicated miRNAs is shown. The heatmap shows high expression in green and low expression in red. b Fold changes (FC) of miRNA expression is indicated when comparing melanocytes (ME), primary melanomas (PM), lymph node metastases (LNM) and distant cutaneous metastases (DCM), respectively. c Melanoma cells were transfected with miR-150 and seeded at low density. Colony formation was counted by light microscopy. d miR-150 was overexpressed in B16V mouse melanoma cells after transfection of the microRNA mimic, and tumor cells were injected into flanks of C57BL6N mice. Tumors were surgically removed after 14 days and weighted. e B16V melanoma cells were transfected using antimiRs against miR-150 or antimiR-control oligos. Mice were sacrificed after 14 days and surgically removed tumors were weighted. f LOX cells were transfected with antimiRs and analyzed for growth rates (here: in the interval 96 h–120 h after transfection)