Fig. 2
From: Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells
Cell viability, spreading and lamellipodium and filopodium formation in HEK293 cells at different concentrations (0–1 μg/μL) of MNPs@SiO2(RITC). a Fluorescence images of cells taken using confocal laser scanning microscopy (CLSM) (LSM710). Green, F-actin; red, MNPs@SiO2(RITC); blue, Hoechst 33342. Scale bar = 20 µm. Lamellipodia and filopodia were indicated by yellow and white arrows. b Viability of cells at 12 h after MNPs@SiO2(RITC) treatment. The experiment is performed in triplicate samples. c Relative area (ratio) of cells on cover slips were taken after 12-h incubation with and without MNPs@SiO2(RITC). The relative area was calculated by dividing the cell area of the treated cells by the cell area of the untreated cells. Data represent the mean ± standard deviation (SD). Cell number (n) > 400. Student’s t-test. * P < 0.05. NS: not significant