Fig. 5
Study on the targeting of CCM and the anti-tumor effect of CCM@MSNs-ISOIM in vitro. A The LCFM images of DSPE-FITC-labeled CCM vesicles were co-cultured with OCI-LY10 cells for 6 h. B Detection of membrane specific protein Pan-cadherin and E-cadherin by Western blotting. C Proliferation inhibition rate of OCI-LY10 cells treated with different concentrations of ISOIM for 24 h and 48 h. (* p < 0.05 compared with 0 μg. mL−1, #p < 0.05 compared with 24 h). D Survival rate of OCI-LY10 cells treated with a) PBS, b) ISOIM, c) MSNs@ISOIM and d) CCM@MSNs-ISOIM for 48 h, respectively. E Representative images of colony formation of OCI-L10 cells treated with a) PBS, b) ISOIM, c) MSNs@ISOIM and d) CCM@MSNs-ISOIM for 14 days. Data are presented as the mean ± SD (n = 3). (* p < 0.05 and ** p < 0.01)