Table 1 List of predicted mRNA targets of the miRNAs
From: Improvement of stem cell-derived exosome release efficiency by surface-modified nanoparticles
miRNA | Predicted target mRNA | Functions and characteristics | Database on ExoCerta | |
|---|---|---|---|---|
Symbol | Full name | |||
mmu-miR-2137 | Gpx4 | Glutathione peroxidase 4 | Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation | o |
Gosr1 | Golgi SNAP receptor complex member 1 | Regulates intracellular reactive oxygen species levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13, and MAPK14) | o | |
Rab4A | Ras-related protein Rab-4A | Protein transport. Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling | o | |
Prrx1 | Paired related homeobox 1 | Enhances DNA-binding activity, and induces genes by growth and differentiation factors | Unknown | |
Pex26 | Peroxisomal biogenesis factor 26 | Peroxisome biogenesis factor 1, also known as PEX1 | Unknown | |
mmu-miR-3473b | Rspry1 | Ring finger and SPRY domain containing 1 | Extracellular region and zinc ion binding | o |
Ephb2 | Eph receptor B2 | Encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins | o | |
Cplx2 | Complexin 2 | Refers to one of a small set of eukaryotic cytoplasmic neuronal proteins that binds to the SNARE protein complex (SNAREpin) with high affinity | o | |
Il-7 | Interleukin 7 | IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells | o | |
Rab11fip1 | RAB11 family interacting protein 1 | Forms a targeting complex that recruits a group of proteins involved in membrane transport to organelles | o | |
mmu-miR-3473e | St6galnac6 | ST6-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 | Modifies ceramides on the cell surface to alter cell–cell or cell-extracellular matrix interactions | o |
Adcy1 | Adenylate cyclase 1 | Regulates various cellular functions via activating protein kinase A-dependent phosphorylation | o | |
Wnk2 | Serine/threonine-protein kinase WNK2 | Plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation | o | |
Sioa1l3 | Signal-induced proliferation-associated 1-like protein 3 | Plays a critical role in epithelial cell morphogenesis, polarity, and adhesion and cytoskeletal organization in the lens | Unknown | |
mmu-miR-5126 | Atp6v1c2 | V-type proton ATPase subunit C 2 | Subunit of the peripheral V1 complex of vacuolar ATPase | o |
CD4 | CD4 molecules | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses | o | |
Pax2 | Paired box 2 | Has a critical role in the development of the urogenital tract, the eyes, and the central nervous system | Unknown | |
Aire | Autoimmune regulator | Expressed by a distinct bone marrow-derived population; induces self-tolerance through a mechanism that does not require regulatory T-cells and is resistant to innate inflammatory stimuli | Unknown | |
mmu-miR-455-3p | Tti1 | TELO2-interacting protein 1 homolog | Phosphorylated at Ser-823 by CK2 following growth factor deprivation, leading to its subsequent ubiquitination by the SCF (FBXO9) complex | o |
Hk3 | Hexokinase 3 | HK3 functions to protect the cell against apoptosis. Overexpression of HK3 has resulted in increased ATP levels, decreased reactive oxygen species production, attenuated reduction in the mitochondrial membrane potential, and enhanced mitochondrial biogenesis | o | |
Sar1a | Secretion associated Ras-related GTPase 1A | Involved in membrane trafficking. It is a monomeric small GTPase found in COPI vesicles. It regulates the assembly and disassembly of COPII coats | o | |
Fgf4 | Fibroblast growth factor 4 | Possesses broad mitogenic and cell survival activities and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, and tissue repair | Unknown | |
Pex5 | Peroxisomal biogenesis factor 5 | Targeting sequence involved in the specific transport of molecules for oxidation inside the peroxisome | Unknown | |
mmu-miR-877-5p | Gpx1 | Glutathione peroxidase 1 | Belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide by glutathione and, thereby, protects cells against oxidative damage | o |
Aak1 | AP2 associated kinase 1 | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2), which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis | Unknown | |
Wnk3 | WNK lysine deficient protein kinase 3 | Plays a role in the increase of cell survival in a caspase 3-dependent pathway as a positive regulator of the transcellular Ca2+ transport pathway | Unknown | |
Rab26 | RAB26, member RAS oncogene family | Important regulators of vesicular fusion and trafficking. The RAB family of small G proteins regulates intercellular vesicle trafficking, including exocytosis, endocytosis, and recycling | Unknown | |
Sult1e1 | Sulfotransferase family 1E, member 1 | Catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds | Unknown | |
mmu-miR-149-3p | Apoc2 | Apolipoprotein C-II | Extracellular region or secreted. Hydrolyzes triglycerides and thus provides free fatty acids for cells | o |
Rab11fip5 | Rab11 family interacting protein 5 | Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane | Unknown | |
Leng8 | Leukocyte receptor cluster member 8 | Predicted using known transcription factor binding site motifs | Unknown | |
Galnt17 | Polypeptide N-acetylgalactosaminyl transferase 17 | Serve a variety of structural and functional roles in membrane and secreted proteins | Unknown | |