Fig. 4
From: Microtubule cytoskeleton-disrupting activity of MWCNTs: applications in cancer treatment
Intracellular MWCNTs prompt ectopic microtubule polymerization. a Diagram of the microtubule cold-depolymerization experiment. b The microtubule cytoskeleton (labeled in green) is cold-sensible. Upon cell exposure to 4 °C, the microtubules depolymerize and tubulin is only visible at the centrosome (red arrows). Upon return into permissive conditions (37 °C), microtubules regrow from the centrosomes (top, right, arrows). If cells were previous exposed to MWCNTs, tubulin re-polymerizes ectopically all over the cytoplasm. The figure is partially adapted from ref. [31]