Fig. 5

Head-to-head comparison of penetration of mTHPC-loaded nanovesicles in F5M5 spheroids. a The typical fluorescence images of cryosections of co-culture (F5M5) spheroids at day 5 post-seeding after incubation with Foslip^®, mTHPC-DCL and mTHPC-EVs for 24 h. mTHPC fluorescence was measured at equal exposure time for all NPs and is displayed in pseudo-colors. The concentration of mTHPC was 4.5 µM. Serum concentration – 6%. Scale bar – 100 µm. b The quantification analysis of mTHPC fluorescence intensity in F5M5 spheroid’ core (red circles) after 24 h incubation with Foslip^®, mTHPC-DCL and mTHPC-EVs. Data presented as mean ± SD [n = 4–7; *p < 0.05; *p < 0.01 and *p < 0.001, using ANOVA]