Fig. 6

The quantification analysis of NPs penetration in spheroids at 24 h incubation. a–c The fluorescence profiles, as a function of distance from the spheroid periphery. Fluorescence intensity is presented as mean ± SD. d–f The cumulative uptake curves of mTHPC-loaded NPs in F5 (blue), F5M2 (green), and F5M5 (red) spheroids. The curve is presented as a mean of 4–10 profiles. g–i The mid penetration depth (d_1/2) of NPs in F5 ( ), F5M2 ( ), and F5M5 ( ) spheroids. Data presented as mean ± SD (box) [n = 4–10; *p < 0.05, using ANOVA]