Fig. 8

Cellular uptake of mTHPC-loaded nanovesicles in co-culture spheroids. Kinetics of mean fluorescence intensity (MFI) of mTHPC histograms in F5 ( ), F5M2 ( ) and F5M5 ( ) spheroid individual cells after 3 h, 6 h, 15 h and 24 h incubation with (a) Foslip^®, (b) mTHPC-DCL and (c) mTHPC-EVs. d–f Coefficients of variation of flow cytometry histograms of spheroids in the function of time. The mTHPC concentration was 4.5 µM. Serum concentration – 6%. Data presented as mean ± SD [n = 3–10; *p < 0.05; *p < 0.01 and *p < 0.001, using ANOVA for each time point]