Fig. 9

Head-to-head comparison of cellular uptake of mTHPC-loaded nanovesicles in spheroids. a Typical flow cytometry histograms of disassociated FaDu spheroids treated with Foslip^® (blue), mTHPC-DCL (cyan) and mTHPC-EVs (red) at 24 h post-incubation. b MFI and (c) coefficients of variation (CV) of flow cytometry histograms at 24 h of spheroids exposed to Foslip^® ( ), mTHPC-DCL ( ) and mTHPC-EVs ( ). The mTHPC concentration was 4.5 µM. Serum concentration – 6%. Data presented as mean ± SD [n = 5–10; *p < 0.05; *p < 0.01 and *p < 0.001, using ANOVA for each type of spheroids]