Fig. 2

Synaptosomes can interact with cells. a Histogram of fluorescence intensity and fluorescence image of synaptosomes stained with Nile Red (Synap Nile Red) and related cartoon. b Histogram of fluorescence intensity and fluorescence image of synaptosomes stained with CTX-FITC (Synap CTX-FITC) and related cartoon. c Cartoon of neuronal cells incubated with synaptosomes stained with Nile Red or CTX-FITC. d Histogram of fluorescence intensity of LAN5 cells incubated with different doses of synaptosomes labeled with Nile Red (Synap Nile Red 5–10 and 20 µl). e Representative fluorescence images of Synap NileRed and LAN5 cells interaction, nuclei stained with Hoechst 33342. f Fluorescent intensity histogram of LAN5 cells incubated with different doses of synaptosomes labeled with CTX-FITC (Synap CTX-FITC 5–10 and 20 µl). g Representative fluorescence images of Synap CTX-FITC and LAN5 cells interaction. Nuclei were stained with Hoechst 33342. h Schematic representation of the localization of Rat synaptophysin (Rat SyP) (red) and Human synaptophysin (Human SyP) (yellow) before and after administration of synaptosomes in LAN5 cell. i Western blot analysis of proteins extracted from LAN5 cells untreated (Control) or treated with different doses of synaptosomes (5–10–20 µl) (Lan5 + synap), and from synaptosomes incubated with anti-Synaptophysin (SyP). j Relative quantification of Rat synaptophysin (Rat SyP) immunoreactive band (36 kDa). The uniformity of gel loading was confirmed by using β-actin as a standard. *P < 0.05, **P < 0.02 vs Control. Scale bar 20 µm. Synap 5–10 and 20 µl; correspond to concentration of 2.5 × 10⁷; 5.1 × 10⁷; 10.2 × 10⁷ particles/100 µl respectively)