Fig. 4

Synaptosomes–cell interaction depends on specific proteins. a Schematic representation of synaptosomes labeled with Nile Red and incubated with trypsin (Synap-NileRed-Tryp) before incubation with LAN5 cells. b Histogram of fluorescence intensity for LAN5 cells incubated with Synap-NileRed-Tryp and Synap-NileRed at different doses (10–20 µl, that correspond to concentration of 5.1 × 10⁷; 10.2 × 10⁷ particles/100 µl respectively) and for the relative control (untreated cells). c Representative fluorescence images for LAN5 cells incubated with Synap-NileRed and Synap-NileRed-Tryp. Nuclei were stained with Hoechst 33342. **P < 0.02. d Representative fluorescence images of LAN5, A549 and HepG2 cells after incubation with Nile Red labeled Synaptosomes (concentration of 10.2 × 10⁷ particles/100 µl) Nuclei were stained with Hoechst 33,342. e Histogram of the relative fluorescence intensity for the three types of cells. **P < 0.02 vs LAN5. Scale bar 20 µm