Fig. 5

Synaptosomes contain vital mitochondria. a Western blotting analysis of proteins extracted from total homogenate (Total) and synaptosomal fraction (Synapt) incubated with antibodies against the mitochondrial proteins Opa1, Fis1 and TOM40. b Red fluorescence intensity for synaptosomes incubated with or without (Synap) JC1 and for synaptosomes treated with CCCp and incubated with JC-1 (Synap-JC1-CCCp) (positive control). c Representative JC1 fluorescence images of synaptosomes incubated with or without (Synap-JC1) CCCp. Cryopreservation of Synaptosomes d Schematic representation of the synaptosomes freezing procedure. e Table of the values of mean diameter and zeta potential of fresh and thawed synaptosomes. f Red fluorescence intensity of fresh (fresh Synap-JC1) and thawed (thawed Synap-JC1) synaptosomes incubated with JC-1. g Representative JC1 red fluorescence images of fresh and thawed synaptosomes incubated with JC-1. **P < 0.02 vs Control. Scale bar 20 µm