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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Double attack strategy for leukemia using a pre-targeting bispecific antibody (CD20 Ab-mPEG scFv) and actively attracting PEGylated liposomal doxorubicin to enhance anti-tumor activity

Fig. 2

Characterization of CD20 Ab-mPEG scFv, CD20 Ab-DNS scFv and CD20 mAb. a The gene constructs of BsAbs are composed of a signal peptide (SP), the anti-CD20 light chain (LC), an internal ribosome entry site (IRES), the anti-CD20 heavy chain (HC), a flexible linker peptide (LK) and an anti-PEG scFv (CD20 Ab-mPEG scFv) or control anti-DNS scFv (CD20 Ab-DNS scFv). b The SDS-PAGE of purified CD20 Ab (Ab), CD20 Ab-DNS scFv (DNS) and CD20 Ab-mPEG scFv (PEG) under reducing conditions. M: PageRuler pre-stained protein ladder (Fermentas). c The CD20 functions and d bispecific binding (CD20 and mPEG) function of BsAbs on Raji (CD20+) cells were detected by anti-Fc-FITC or PEGylated lipo-DiD respectively via flow cytometry

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