Fig. 8
a Line 1 (green) represents the calcein 3 weeks prior to sacrificing the mice. Line 2 (red) represents the alizarin red 3 days prior to sacrificing the mice. Line 3 represents the combination of the two florescence lines. b Quantitative analysis of the two florescence lines. c, d illustrates the results of HE staining and Masson staining for the investigation of the bone and collagen components of the control, CS, CePO4/CS, and CePO4/CS/GO groups. Figure 8c â‘ a large number of osteoblasts are adhered to the surface of the bone matrix. Figure 8c â‘¡ a large number of macrophages are aggregated near the material. Figure 8c â‘¢ new blood vessels formed by vascular endothelial cells. Figure 8e immunohistochemical analysis of VEGF of CePO4/CS and CePO4/CS/GO groups