Fig. 2

pRNA-EGFRapt-siXBP1 NPs cell uptake, specific binding to tumor cells in vitro. a, b Cell binding is assessed by flow cytometry. c Confocal images showing efficient binding and internalization into MDA-MB-231 cells. Green: cell membrane; blue: nuclei; and red: RNA nanoparticles. Scale bars, 10 µm. d Representative gating strategies in mixture of MDA-MB-231 and MCF10A cells. e Cell specific binding is assessed by flow cytometry. f pRNA-EGFRapt-siXBP1 NPs binding efficiency were evaluated. g MFI of Cy5 positive cells