Fig. 4

pRNA-EGFRapt-siXBP1 NPs specifically bind to tumor cells and efficiently silence XBP1 expression in vivo. a Pharmacokinetic study of pRNA-EGFRapt-siXBP1. b Quantitative RT-PCR analysis of XBP1 expression in MDA-MB-231 xenograft tumor. Data are presented relative to β-actin. n = 3. c Western blot analysis of XBP1s and XBP1u expression in MDA-MB-231 tumor mouse. d Biodistribution of Cy5-labeled pRNA-EGFRapt-siXBP1 NPs 16 h post intravenously injection. e Confocal microscopic images of tumor sections from mice injected intravenously with pRNA-EGFRapt-siXBP1 or control NPs without EGFR aptamer. Red, Cy5-labelled pRNA; green, Alexa488-wheat germ agglutinin-labelled cell membrane; blue, nuclear staining with DAPI. Scale bars, 20 µm and 7 µm, respectively