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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Surface charge modulation of rifampicin-loaded PLA nanoparticles to improve antibiotic delivery in Staphylococcus aureus biofilms

Fig. 6

Migration of NPs and PLL-coated NPs in S. aureus biofilms. Time-lapse CLSM micrographs of NP-Dy650 (a) and NP-Dy650-PLL (b) diffusion (in red) into an 18 µm layer thickness (between + 10 and + 28 µm from the plate bottom) of Syto9-stained S. aureus biofilm (in green) after 8 (left), 44 (center) and 80 (right) min, with bottom and side views. c Kinetic of red-fluorescence NP diffusion into an 18 µm layer thickness of Syto9-stained S. aureus biofilm. d CLSM micrographs of NP-Dy650 (up) and NP-Dy650-PLL (down) after 18 h diffusion into an 18 µm layer thickness of Syto9-stained S. aureus biofilm with bottom and side views. e Red fluorescence intensity of NPs into an 18 µm layer thickness of Syto9-stained S. aureus biofilm after 18 h diffusion. Each picture represents data obtained with three replicates in three independent experiments. Values on graphs (c) and (e) are means ± SD of three replicates for one representative experiment out of three independent ones. Unpaired t-test, * p < 0.05

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