Fig. 10
Cell cycle arrest analysis of HT-29 cells treated with different formulations of DOX and CUR. a Flow cytometry evaluation of DNA content in HT-29 cells after incubation with various formulations of drugs including free drugs, single/dual drug loaded-hydrogels, and nanogels for 48 h in concentrations around their IC50 values. b The proportion of cell cycle phase (%) and DNA distribution percentages in different cell cycle phases (subG1, G0-G1, S, and G2/M) for various formulations after DAPI staining in HT-29 cells. One-way ANOVA, followed by Tukey's HSD analysis, was used to determine p-values for different phases of the cell cycle. The difference was considered significant at *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001