Fig. 1
From: Decrease in membrane fluidity and traction force induced by silica-coated magnetic nanoparticles
Laurdan GP images and GP frequency distributions of MNPs@SiO2(RITC)-treated HEK293 cells. a Schematic representation of laurdan usage for measuring the GP value of membranes. b Merged DIC and psuedo-coloured GP images (upper panel) of HEK293 cells. MNPs@SiO2(RITC) distributions are shown in each lower panel. GP distributions ranged from − 1.0 to 1.0. Scale bar = 2.5 µm. c GP frequency distributions of cells. GP values of each pixel are represented as dots and were fitted to Gaussian distributions. d Total GP values. Data represent the mean ± SD of 3 independent experiments (N = 10). e Evaluation of peroxidised lipids using ferrous thiocyanate. The intensity of ferrous thiocyanate alone was used as the blank. Data represent the mean ± SD of 3 independent experiments. *p < 0.05 vs untreated control, #p < 0.05 compared between 0.1 and 1.0 µg/µL MNPs@SiO2(RITC)-treated cells