Fig. 3
From: Decrease in membrane fluidity and traction force induced by silica-coated magnetic nanoparticles
Evaluation of the movement of MNPs@SiO2(RITC)-treated HEK293 cells. a Representative images of the scratch assay and quantitative image analysis. Images of the initial scratched (0 h) layer are shown in the upper panels. Images of HEK293 cells after treatment with MNPs@SiO2(RITC) for 16 h are shown in the lower panels. Scale bar = 100 μm. Quantitative image analysis of migrated cells in MNPs@SiO2(RITC)-treated HEK293 cells is shown in the bar graph. b Representative images of HEK293 cells and quantitative image analysis of the invasion assay results after treatment with MNPs@SiO2(RITC) for 12 h. Red, MNPs@SiO2(RITC); blue, Hoechst 33,342. Scale bar = 20 μm. Quantitative image analysis of invaded MNPs@SiO2(RITC)-treated HEK293 cells is shown in the bar graph. Individual cell tracking analysis for travel distance (c) and velocity (d) of MNPs@SiO2(RITC)-treated HEK293 cells at 6, 12, and 24 h. Data represent mean ± SD of 3 independent experiments. *p < 0.05 vs. untreated control, #p < 0.05 compared between 0.1 and 1.0 µg/µL MNPs@SiO2(RITC)-treated cells