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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Fig. 2

Cell-type specific uptake of B16F10-EVs. a B16F10, RAW264, HEK293T, MC38, and NIH3T3 cells were treated with DiD-labeled B16F10 EVs for 6 or 24 h and analyzed by flow cytometry. B16F10 cells were treated with DiD-labeled EVs isolated from either MC-38, RAW264, B16F10 or HEK293T cells and analyzed by b flow cytometry and c confocal microscopy after 6 or 24 h of incubation. For confocal microscopy, DiD-labeled EVs are shown in red, DAPI in blue and membrane staining with DiO in green. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05 (respectively to control, mean ± SEM; n = 4). Scales bar are 50 µm

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