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Fig.3 | Journal of Nanobiotechnology

Fig.3

From: Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Fig.3

Uptake and cytotoxicity of AuNP-PEG-FA. a UV–Vis spectra of AuNP, AuNP-PEG and AuNP-PEG-FA (mean of n = 3). b Representative TEM micrograph of AuNP and size distribution obtained from 100 particles. Scale bar is 200 nm. c MTS assay of B16F10 cells incubated with different concentrations of AuNP-PEG-FA for 24 h. Ctrl corresponds to cells in the culture medium without AuNPs and SDS corresponds to cells incubated with 2% SDS. d Gold content by NAA of B16F10 cells incubated with 1 nM AuNP-PEG-FA for different periods of time. e Flow cytometry analysis of confluent B16F10 cells incubated with AuNP-PEG-FA prior to EV isolation. ****P < 0.0001; ***P < 0.001 (respect to control, mean ± SEM; n = 3)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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