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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Fig. 4

Internalization of AuNP-PEG-FA and secretion from B16F10 cells. B16F10 cells were transfected to express CD63-RFP (magenta) and pulsed with AuNP-PEG-FA (cyan) for 30 min. Then, the cells were incubated with AuNP-PEG-FA in combination with the endocytosis marker Tf-488 (yellow) and imaged in a confocal microscope using time lapse live image acquisition mode. The colocalization of AuNP-PEG-FA with Tf-488 and CD63-RFP is indicated by white and red arrows, respectively. Representative time points are shown. Colocalization of AuNP-PEG-FA with CD63 (dashed blue square) is shown at higher magnification (zoom). Scale bars are 10 μm

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