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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Fig. 5

Isolation and uptake of double-labeled EVs by B16F10 cells. Representative a TEM and b Cryo-TEM micrographs of EV-AuNP; scales bars are both 200 nm. c NTA of EV and EV-AuNP. d Western blot of cell lysates (CL) and EVs. e NAA and f UV–Vis spectra of control EVs and EV-AuNP. B16F10 cells were treated with either PBS (control), AuNP-PEG-FA (AuNP), DiR-labeled EVs (EV) or DiR-labeled EV-AuNP (EV-AuNP) for 6 or 24 h. Flow cytometry analysis of g DiR fluorescence intensity and h NAA of internalized gold after incubation. i Percentage of total EV-AuNP internalization determined by fluorescence and gold quantification. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05 (respectively to control, mean ± SEM; n = 3)

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