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Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Fig. 7

Detection of gold labeled EVs in small metastatic nodules. C57BL/6 mice were injected with 2 × 105 of B16F10 cells to produce lung metastasis and then injected day 19 with either DiR EVs (EV), DiR EV-AuNP (EV-AuNP) or AuNP-PEG-FA (AuNP). On day 20, animals were necropsied, and lungs were analyzed by gold nucleation detection. Histological gold detection in the lungs of mice inoculated with a DiR EVs, b DiR EV-AuNP and c AuNP-PEG-FA. In all cases, dotted squares show magnified images of sections highlighted to the left by red squares, while red arrows indicate the gold nucleation sites (GN). A: Alveolus parenchyma, M: metastatic melanoma nodules and V are the Vessels. d Gold nucleation was quantified and graphed for the areas adjacent to the blood vessels and in the lung metastasis nodules. Data are means ± SEM from n = 8 samples per condition. ****P < 0.0001; **P < 0.01; *P < 0.05

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