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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Development of elastin-like polypeptide for targeted specific gene delivery in vivo

Fig. 6

In vivo gene silencing by siRNA/ELP variant complexes. a For in vivo gene silencing, siRNA-1 (5 µg) was encapsulated using 200 µM of ELP variants and administered by intravenous injection to Balb/c wild mice (n = 10) bearing a 4T1 tumor daily for 6 days. Time-dependent reductions in bioluminescence intensities of subcutaneous tumors were determined by IVIS imaging. b Quantification of tumor bioluminescence intensities at different times (n = 10). *P < 0.05 (Student’s t-test), significant difference for Tat-A1E28 or Tat-A4V48 compared with free-siRNA. c After the 6-days treatment period tumor tissues were excised and tissue sections were stained with anti-Luc antibody. After nuclear staining with DAPI, tissue sections were observed under a confocal microscope. Hoechst, blue; and anti-Luc antibody, green. The confocal images are representative of three experiments (Scale bar, 20 μm)

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