Table 2 The PCR cycling conditions
Steps | Temp. (°C) | Time (min) | Cycles |
|---|---|---|---|
Initial denaturation | 95 | 10 | 1 |
Denaturation | 94 | 1 | 42 |
Annealing | 45 | 1.5 | Â |
Extension | 72 | 1.5 | Â |
Final extension | 72 | 3 | 1 |
Steps | Temp. (°C) | Time (min) | Cycles |
|---|---|---|---|
Initial denaturation | 95 | 10 | 1 |
Denaturation | 94 | 1 | 42 |
Annealing | 45 | 1.5 | Â |
Extension | 72 | 1.5 | Â |
Final extension | 72 | 3 | 1 |