Fig. 3

Cellular uptake of nanovaccine by carp macrophage in vitro. a Uptake of FITC labelled nannovaccines by carp macrophage in vitro. b Mean florescence intensity of cell uptake capability. c Representative confocal microscopic images of carp macrophage incubated with G, MG, SWCNTs-G, and SWCNTs-MG respectively. Vaccines were labeled with FITC (green channel, white arrows), respectively; The cell nucleus was labeled with DAPI (blue channel); The mannose receptor was labeled with Cy3 (red channel)