Fig. 2

Delivery efficiency and toxicity of TAP/2′-OMePS E50 complexes in a C2C12E50 cell line determined by fluorescence microscopy and fluorescence-activated cell sorting (FACS) analysis. a Representative fluorescence images of 2′-OMePS E50-induced exon-skipping in C2C12E50 cell line. (The images were taken 4-day after treatment. Original magnification ×200; scale bar 500 µm). b Statistical date of TE of 2′-OMePS E50 formulated with TAP (Student’s t-test, *p ≤ 0.05 compared with 2′-OMePS E50 only). c Cell viability (Student’s t-test, *p ≤ 0.05 compared with untreated cell as control). In this test, 2 µg 2′-OMePS E50 was formulated with TAPs (5 and 10 µg), and PEI 25 (5 µg), LF-2k (4 µg) formulated as comparison in 0.5 mL 10% FBS-DMEM medium, respectively. The results are presented as the mean ± SD in triplicate