Fig. 7
From: Triazine-cored polymeric vectors for antisense oligonucleotide delivery in vitro and in vivo
Restoration of dystrophin in tibialis anterior muscles of mdx mice (aged 4–5 weeks) 2 weeks after intramuscular injection with 20 μg polymer formulated 2′-OMePS (5 μg) in 40 μL saline. Muscles treated with 2′-OMePS only was used as controls. a Dystrophin was detected by immunohistochemistry with rabbit polyclonal antibody P7 against dystrophin. Blue nuclear staining with 4, 6-diamidino-2-phenylindole (original magnification ×200; scale bar 200 μm). b The percentage of dystrophin-positive fibers (mean ± SD, n = 5, Two-tailed t-test, *p ≤ 0.05 compared with 2′-OMePS)