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Table 1 Primer sequences of amplification VHH genes for recombinant plasmids pcDNA3.1-Nbs-hFc construction

From: Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein

Primer

Sequences

Nbs-F

5′-CAGGGATCCCAGGTGCAGCTGGTGGAGTC-3′

Nbs-R

5′-CGCCTCGAGTGAGGAGACGGTGACCTGGG-3′

biNbs-R1

5′-TGAACCGCCTCCACCGCTGCCGCCTCCGCCTGAGGAGACGGTGACCTGGG-3′

biNbs-F2

5′-GGTGGAGGCGGTTCAGGAGGTGGCGGATCTCAGGTGCAGCTGGTGGAGTC-3′

triNbs-R32

5′-AGATCCGCCACCTCCGCTGCCGCCTCCGCCTGAGGAGACGGTGACCTGGG-3′

triNbs-F3

5′-GGAGGTGGCGGATCTGGTGGAGGCGGTTCACAGGTGCAGCTGGTGGAGTC-3′

  1. Underline indicates position of restriction enzyme sites