Table 2 Enrichment of phage particles against SARS-CoV-2-spike and -RBD specific nanobodies during four rounds of panning
Round of screening | Input (pfu/well) | P output (pfu/well) | N output (pfu/well) | Recovery (P/input) | P/N | ||||
|---|---|---|---|---|---|---|---|---|---|
S | RBD | S | RBD | S | RBD | S | RBD | ||
1st round | 1.0 × 1011 | 1.5 × 104 | 1.4 × 104 | 8.4 × 103 | 9.1 × 103 | 1.5 × 10− 7 | 1.4 × 10− 7 | 17.8 | 1.5 |
2nd round | 1.0 × 1011 | 6.5 × 105 | 3.4 × 105 | 3.4 × 103 | 2.9 × 103 | 6.5 × 10− 6 | 3.4 × 10− 6 | 1.9 × 102 | 1.2 × 102 |
3rd round | 1.0 × 1011 | 2.6 × 107 | 1.9 × 107 | 4.5 × 104 | 3.8 × 104 | 2.6 × 10− 4 | 1.9 × 10− 4 | 5.7 × 102 | 5.0 × 102 |
4th round | 1.0 × 1011 | 5.4 × 108 | 5.2 × 108 | 3.6 × 105 | 2.7 × 105 | 5.4 × 10− 2 | 5.2 × 10− 2 | 1.5 × 103 | 1.8 × 103 |