Fig. 6

Co-culture cell viability and chromosomal damage was evaluated by CBPI and the in vitro CBMN assay. Co-culture models exposed to neutral-FLG (a), amine-FLG (b), carboxyl-FLG (c) and CB (d) showed no significant cytotoxicity, each ENM induced a genotoxic response at concentrations of 20-50 µg/ml with only carboxyl-FLG not causing genotoxicity at 10 µg/ml. Results were considered significant (*) when p < 0.05. MMC (0.01 µg/ml) demonstrated a 7.2% Mn/BN frequency. Data is presented as the average value ± the standard deviation (SD), (N = 3)