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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Modular vaccine platform based on the norovirus-like particle

Fig. 2

Conjugation of norovirus-like particles. a Silver-stained SDS-PAGE gel of the vaccine components. The noro-VLP appears as a double band between the 70 and 50 kDa markers. All wells contain approximately 250 ng of protein. b Western blot analysis of noro-VLP decorated with the influenza antigens. The HisTag in the N-termini of SpyCatcher fusion proteins is recognized by mouse anti-HisTag antibody. c Dynamic light scattering analysis of SpyTag-noro-VLP and SpyTag-noro-VLP decorated with M2e and HA2. Numerical data shown in Additional file 1: Figure S4. Representative transmission electron microscopy pictures with arithmetic means of equivalent circle diameters ± standard deviation of d native noro-VLP (n = 114), e SpyTag-noro-VLP (n = 112) and noro-VLP decorated with f M2e (n = 109) and g HA2 (n = 274). The scale bars are all 200 nm. 60,000× magnification was used for these pictures and analysis

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