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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Fusion expression of nanobodies specific for the insecticide fipronil on magnetosomes in Magnetospirillum gryphiswaldense MSR-1

Fig. 1

Construction of recombinant strains biomineralizing Nb-magnetosomes. a The schematic diagram of recombinant strain construction. b Colony PCR of CF. genes in group 1 were amplified by using Fip-F and Fip-R (left to right: marker, negative control, Nb F1, and CF); genes in group 2 were amplified by P1 and P3 (left to right: marker, negative control, WT MSR-1, and CF); genes in group 3 were amplified by P2 and P4 (left to right: marker, negative control, WT MSR-1, and CF). c Colony PCR of CF+ . The first lane and the last lane were negative control and marker, respectively; the rest of lanes were sampling. Primers used were pBBR-F and pBBR-R. d Colony PCR of CFFF. The first and the second lane were marker and negative control, respectively; the rest of lanes were sampling. Primers used were mamF and Fip-R. All primers were listed in Additional file 1: Table S2

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