Fig. 3

Inhibition of hemolysins secreted by GAS and GGS supernatants by Trypan Blue. Trypan Blue, a selective inhibitor of SLS, provides partial protection against hemolytic activities of GGS supernatants and no protection against GAS supernatants (a, b). However, the full neutralization of GAS/GGS hemolysins is achieved by the combination of Trypan Blue with Ch:Sm-liposomes (c). Please note that in c Ch:Sm-liposomes (invariable amount; 2 µg) are present in all experimental conditions (varying amounts of Trypan Blue). As a result, GAS supernatants display already diminished hemolytic activity (~ 50% hemolysis) even in the absence of Trypan Blue, whereas GGS strains are fully hemolytic at these conditions. Error bars = Mean ± SD. N ≥ 3