Table 1 Enrichment of nanobodies against PCV2-Cap protein from the phages during three rounds panning
Round of panning | Input phage (PFU/well) | Output phage/P (PFU/well) | PBS output/N (PFU/well) | Recovery rate/(P/Input) | Enrichment(P/N) |
|---|---|---|---|---|---|
1st | 5 × 1010 | 3.9 × 105 | 7.2 × 104 | 7.8 × 10− 6 | 5.4 |
2nd | 5 × 1010 | 5.5 × 105 | 7 × 104 | 1.1 × 10− 5 | 7.9 |
3rd | 5 × 1010 | 3 × 106 | 2 × 103 | 6 × 10− 5 | 1.5 × 103 |