Skip to main content

Table 2 Determination of the optimal coating amount of PCV2-Cap protein and the optimal dilution of PCV2-Nb15-HRP fusion protein

From: A nanobody‐horseradish peroxidase fusion protein‐based competitive ELISA for rapid detection of antibodies against porcine circovirus type 2

Dilution of PCV2-Nb15-HRP

The OD450nm value of different amounts of PCV2-Cap protein (µg/mL)

0.5

1

2

4

8

1:100

2.109 ± 0.062

2.283 ± 0.004

2.536 ± 0.026

2.688 ± 0.049

2.874 ± 0.020

1:200

1.620 ± 0.088

1.962 ± 0.010

2.014 ± 0.034

2.316 ± 0.173

2.804 ± 0.015

1:400

1.170 ± 0.011

1.304 ± 0.018

1.435 ± 0.037

1.684 ± 0.019

2.490 ± 0.014

1:800

0.451 ± 0.074

1.098 ± 0.001

1.193 ± 0.024

1.35 ± 0.038

1.727 ± 0.029

1:1600

0.264 ± 0.038

0.477 ± 0.009

0.522 ± 0.003

0.686 ± 0.005

1.165 ± 0.053

1:3200

0.180 ± 0.020

0.209 ± 0.008

0.215 ± 0.004

0.396 ± 0.011

0.634 ± 0.011

  1. The italics represent the selected concentration of antigen and the dilution of nanobody