Fig. 2
Characterization, In vitro Cytotoxicity Assays, H2S Release Assessment, and Cellular Uptake of DATS@MION-PEG-LF. a TEM and b enlarged TEM images. c The DLS results demonstrated a narrow size distribution of the nanoparticles. d Neuron and e cardiomyocytes cell viability after incubated for 24 h in different concentrations of DATS@MION-PEG-LF (mean ± SEM, n = 3). f Release of H2S from DATS@MION-PEG-LF in PBS solution. DATS@MION-PEG-LF (20, 10, and 5 µg/mL) with GSH (2 mM) (pH 7.4, 37 oC). g DATS@MION-PEG-LF (10 µg/mL) with GSH (2 mM) at pH 7.4, 6.5, and 8.0 (37 oC). h DATS@MION-PEG-LF (10 µg/mL) with GSH (2 mM) at 37, 20, and 4 oC (pH 7.4). Fluoresce microscopic images after 4 h incubation with DiR@MION-PEG-LF (50 µg/mL) in neurons (i) and cardiomyocytes (j). Red: DiR@MION-PEG-LF, Green: cytoplasm stained by cTnT (cardiomyocytes) and MAP-2 (neurons), Blue: nucleus stained by DAPI