Fig. 7

DATS@MION-PEG-LF protects ischemic heart after CA/CPR. TUNEL staining detected cardiomyocytes apoptosis at 24 h after CA/CPR. Nuclei with green staining indicate TUNEL positive cells (200×) (a); the percentage of TUNEL positive cells to total cardiomyocytes in individual groups (b); myeloperoxidase (MPO) activities (c), superoxide dismutase (SOD) activities (d), catalase (CAT) activities (e), and levels of malonydialdehyde (MDA) contents (f) were measured in heart tissue at 24 h after CA/CPR. The levels of Capase-3, Bcl-2, and Bax in myocardium were tested using western blot assay (g); the intensity of each band was quantified by densitometry, and data were normalized to the Actin signal (h–j). *: P < 0.05 compared with the Vehicle Group (mean ± SEM, n = 6)